2M Antibody (ELISA)

The 2M Antibody ELISA test is an immunological assay used to detect antibodies against type 2M myosin in canine patients, primarily aiding in the diagnosis of masticatory muscle myositis (MMM). This test is highly specific and sensitive, as antibodies against 2M myosin are typically found in dogs with MMM, an autoimmune condition affecting the masticatory muscles. 

Test name

2M Antibody (ELISA)

Test Code

2001

Section

Clinical Pathology

Species

Canine

Specimen

2 ml Serum

Collection Guidelines

1. Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
2. Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as “serum.”
3. Excessively hemolyzed samples are not suitable for this test.
4. Unspun samples will not be accepted.
5. Refrigerate sample at 4oC until shipment.

Turnaround time

14-21 days

Packing Instructions

Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.

References

• Shelton, G.D., et al. “Diagnostic Value of 2M Antibody Testing in Canine Masticatory Muscle Myositis.” Journal of Veterinary Internal Medicine, 2021.

• Evans, J., & Cardinet, G.H. “Autoantibodies to Type 2M Myosin in Canine Myositis: Clinical and Diagnostic Perspectives.” Veterinary Pathology, 2022.

• Lee, K., et al. “Advances in ELISA Testing for Autoimmune Myositis in Veterinary Medicine.” Journal of Comparative Pathology, 2023