Brucella canis (IFA)
The Brucella canis IFA test detects antibodies against Brucella canis, the bacterium responsible for canine brucellosis, using the indirect fluorescent antibody (IFA). Canine brucellosis is a contagious bacterial infection that affects reproductive health, causing infertility, abortion, and other reproductive disorders in dogs. The presence of antibodies in the blood indicates exposure to B. canis and supports the diagnosis of infection, helping to identify carriers and manage disease spread, particularly in breeding populations.
Additionally, this test plays a critical role in pet travel compliance, as some countries require proof of a negative result for Brucella canis as part of their import regulations.
Test name
Brucella canis (IFA)
Test Code
4006
Section
Immunodiagnostics
Species
Canine
Specimen
2 ml Serum
Collection Guidelines
- Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
- Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as “serum.”
- Excessively hemolyzed samples are not suitable for this test.
- Unspun samples will not be accepted.
- Refrigerate sample at 4oC until shipment.
Turnaround time
14-21 days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Wanke, M.M. “Canine Brucellosis: An Update on Diagnostic Approaches and Prevention.” Journal of Veterinary Internal Medicine, 2021.
- Carmichael, L.E., et al. “Diagnosis and Control of Brucella canis Infections in Dogs.” Veterinary Clinics of North America: Small Animal Practice, 2022.
- Keid, L.B., et al. “Advances in Brucella canis Testing: Reliability of IFA for Detecting Antibodies.” Journal of Veterinary Diagnostic Investigation, 2023.
