Cryptosporidium sp. (rtPCR) – Equine
The Cryptosporidium sp. rtPCR test is a molecular diagnostic tool used to detect the DNA of Cryptosporidium species, a protozoan parasite responsible for cryptosporidiosis in equines. This highly sensitive and specific test, enabling the accurate identification of Cryptosporidium even in low-infection cases. In horses, cryptosporidiosis can cause gastrointestinal disturbances, including diarrhea, weight loss, and dehydration, particularly in foals or immunocompromised individuals. It may also present subclinically in some animals, acting as a potential source of infection for others, including humans, as the disease is zoonotic. The rtPCR method is particularly valuable for diagnosing cryptosporidiosis in symptomatic horses, monitoring outbreaks, and identifying asymptomatic carriers. Early and precise detection facilitates timely management, including appropriate hydration and supportive care, thereby reducing the spread of infection within herds or equestrian facilities.
Test name
Cryptosporidium sp. (rtPCR) – Equine
Test Code
E112
Section
Molecular diagnostics
Species
Equine
Specimen
5g fecal sample
Collection Guidelines
- Ideally, fecal samples should be taken directly from the rectum. If samples are collected from the ground, they should be taken from the surface of a freshly deposited stool.
- An appropriate sample size is 5g in a leak proof sterile container.
- Preferably submit the sterile container in a zip-locktype plastic bag
Turnaround time
14-21 Days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Xiao, L., et al. “Cryptosporidiosis in Horses: Advances in Diagnosis and Management.” Journal of Veterinary Internal Medicine, 2021.
- Trout, J.M., et al. “Molecular Diagnostics of Cryptosporidium: The Utility of rtPCR in Equine Infections.” Veterinary Clinics of North America: Equine Practice, 2022.
- Grinberg, A., et al. “Real-Time PCR Testing for Cryptosporidium in Equine Patients: A Review of Applications and Outcomes.” Journal of Veterinary Diagnostic Investigation, 2023.
