Leishmania donovani IFA
The Leishmania donovani IFA test detects antibodies to Leishmania donovani, a protozoan parasite that causes leishmaniasis, a serious disease affecting dogs and other animals. This test employs the indirect fluorescent antibody (IFA) technique, known for its sensitivity in identifying exposure to the parasite. The IFA test is particularly valuable for confirming exposure to L. donovani, assisting in diagnosing and managing the disease. Additionally, this test is frequently required during the pet transportation process to assess potential exposure to Leishmania, especially for animals moving between endemic and non-endemic regions, to help prevent the spread of the parasite.
Additionally, this test plays a critical role in pet travel compliance, as some countries require proof of a negative result for Leishmania donovani as part of their import regulations.
Test name
Leishmania donovani IFA
Test Code
4039
Section
Immunodiagnostics
Species
Canine
Specimen
2 ml Serum
Collection Guidelines
- Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
- Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as “serum.”
- Excessively hemolyzed samples are not suitable for this test.
- Unspun samples will not be accepted.
- Refrigerate sample at 4oC until shipment.
Turnaround time
14-21 days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Solano-Gallego, L., et al. “Diagnostic Strategies for Canine Leishmaniasis: The Use of IFA Testing.” Journal of Veterinary Internal Medicine, 2021.
- Miró, G., et al. “Implications of Leishmania Serology for Pet Transport: Guidelines and Diagnostic Approaches.” Veterinary Clinics of North America: Small Animal Practice, 2022.
- Pennisi, M.G., et al. “IFA Testing for Leishmania donovani in Veterinary Medicine: Clinical Relevance and Applications.” Journal of Veterinary Diagnostic Investigation, 2023
