Tick-borne Pathogen Panel (rtPCR)
The Tick-borne Pathogen Panel (rtPCR) uses real-time polymerase chain reaction (rtPCR) to detect DNA from multiple tick-borne pathogens, including species from the Rickettsiales, Borrelia, and Babesia genera. This comprehensive panel is designed to identify a range of pathogens commonly associated with tick-borne diseases in animals, which can present with symptoms like fever, lethargy, joint pain, anemia, and, in severe cases, neurological issues. Specifically, the panel detects Rickettsiales species (Ehrlichia canis, E. chaffeensis, E. ewingii, Rickettsia rickettsii, and Anaplasma phagocytophilum), Borrelia species (B. burgdorferi, B. hermsii, B. parkeri, B. turicatae), and Babesia species (B. gibsoni, B. canis, B. caballi). The high sensitivity of rtPCR allows for early detection, even at low pathogen levels, which is essential for timely diagnosis and intervention.
Test name
Tick-borne Pathogen Panel (rtPCR)
Test Code
5028
Section
Molecular Diagnostics
Species
Canine
Specimen
2 ml EDTA whole blood
Collection Guidelines
Collect an adequate amount of blood in a K3EDTA (purple top tube). Gently mix blood well after collection. Refrigerate sample at 4oC until shipment..
Turnaround time
14-21 days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Neer, T.M., et al. (2022). “Diagnosis and Management of Tick-Borne Infections in Companion Animals.” Veterinary Clinics of North America: Small Animal Practice.
- Sykes, J.E., et al. (2023). “PCR Detection of Tick-Borne Pathogens: A Comprehensive Approach.” Journal of Veterinary Diagnostic Investigation.
- Little, S.E., et al. (2021). “Epidemiology and Clinical Relevance of Tick-Borne Diseases in Dogs and Cats.” Journal of Small Animal Practice.
