Trypsin-Like Immunoreactivity (TLI) – Canine
The Trypsin-Like Immunoreactivity (TLI) test is a specialized diagnostic tool used to assess pancreatic function in dogs, particularly to diagnose exocrine pancreatic insufficiency (EPI). TLI measures serum concentrations of trypsinogen and trypsin, enzymes produced by the pancreas, which are typically present at low levels in healthy dogs. Elevated TLI levels may indicate pancreatitis, while significantly reduced levels are indicative of EPI, a condition where the pancreas fails to produce sufficient digestive enzymes.
Test name
Trypsin-Like Immunoreactivity (TLI) – Canine
Test Code
2023
Section
Clinical Pathology
Species
Canine
Specimen
2 ml Fasting Serum
Collection Guidelines
- Overnight fasting is mandatory.
- Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
- Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as “serum.”
- Excessively hemolyzed samples are not suitable for this test.
- Unspun samples will not be accepted.
- Refrigerate sample at 4oC until shipment.
Turnaround time
14-21 days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Steiner, J.M., et al. “Diagnostic Utility of Serum Trypsin-Like Immunoreactivity in Canine Exocrine Pancreatic Insufficiency.” Journal of Veterinary Internal Medicine, 2022.
- Williams, D.A., & Steiner, J.M. “Advances in the Diagnosis of Canine Pancreatic Disorders: Role of TLI Testing.” Veterinary Clinics of North America: Small Animal Practice, 2021.
- Simpson, K.W., et al. “Pancreatic Function Testing in Dogs: Trypsin-Like Immunoreactivity and Beyond.” Journal of Small Animal Practice, 2023.
