Trypsin-Like Immunoreactivity (TLI) – Feline
The Trypsin-Like Immunoreactivity (TLI) test is a valuable diagnostic tool for evaluating pancreatic function in cats, particularly for diagnosing exocrine pancreatic insufficiency (EPI) and pancreatitis. TLI measures the levels of trypsinogen and trypsin in the blood, which are enzymes produced by the pancreas. In healthy cats, TLI levels remain within a specific range, while low TLI levels indicate EPI due to inadequate enzyme production. Elevated TLI levels, on the other hand, may suggest pancreatitis. Since pancreatic disorders can present subtly in cats, the TLI test is essential for accurate diagnosis, guiding treatment and dietary management for affected felines.
Test name
Trypsin-Like Immunoreactivity (TLI) – Feline
Test Code
2024
Section
Clinical Pathology
Species
Feline
Specimen
2 ml Fasting Serum
Collection Guidelines
- Overnight fasting is mandatory.
- Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
- Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as “serum.”
- Excessively hemolyzed samples are not suitable for this test.
- Unspun samples will not be accepted.
- Refrigerate sample at 4oC until shipment.
Turnaround time
14-21 days
Packing Instructions
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label. Ensure the submittal form is completed with the animal’s information and relevant medical history.
References
- Steiner, J.M., et al. “Evaluation of Serum Trypsin-Like Immunoreactivity in Feline Exocrine Pancreatic Insufficiency.” Journal of Veterinary Internal Medicine, 2022.
- Simpson, K.W., & Steiner, J.M. “Diagnostic Techniques in Feline Pancreatic Disorders: The Role of TLI Testing.” Veterinary Clinics of North America: Small Animal Practice, 2021.
- Forman, M.A., et al. “Trypsin-Like Immunoreactivity in Cats: Diagnostic Utility and Clinical Applications.” Journal of Feline Medicine and Surgery, 2023.
