Acetylcholine Receptor Antibody (RIA)
The Acetylcholine Receptor Antibodies (RIA) test is a radioimmunoassay specifically used to detect antibodies against acetylcholine receptors, aiding in the diagnosis of myasthenia gravis. This autoimmune neuromuscular disease is characterized by the presence of autoantibodies that target acetylcholine receptors at the neuromuscular junction, leading to muscle weakness and fatigue. By measuring the concentration of these antibodies in the blood, the RIA test offers a highly sensitive and specific method for confirming myasthenia gravis, guiding early intervention and appropriate therapeutic management.
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separatio
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Important note: Collect serum prior to initiation of corticosteroid or other immunosuppressive therapy.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
1. Dewey, C.W., et al. "Clinical Utility of Acetylcholine Receptor Antibody Testing in Canine Myasthenia Gravis." Journal of Veterinary Internal Medicine, 2022.
2. Shelton, G.D., & Podell, M. "Advances in Diagnosis and Treatment of Canine Myasthenia Gravis." Veterinary Clinics of North America: Small Animal Practice, 2021.
3. Volk, H.A., et al. "Autoimmune Neuromuscular Disorders in Dogs: Diagnostic and Therapeutic Perspectives." Journal of Veterinary Neurology, 2023.