Amphibian Infectious Disease PCR Panel
The Amphibian Infectious Disease PCR Panel is a comprehensive diagnostic tool designed to detect five significant pathogens responsible for infectious diseases in amphibian populations. This panel employs polymerase chain reaction (PCR) technology for sensitive and specific identification of the following pathogens: Batrachochytrium dendrobatidis (Bd), Batrachochytrium salamandrivorans (Bsal), frog virus 3 (ranavirus), pan-ranavirus, and Perkinsea.
Moist swabs of the affected areas (cloacal swab and fecal swab),
Moist swabs are preferred; it is recommended to add 1-2 drops of sterile saline to the swab and place in a sterile, leak-proof container (e.g. red top tube)
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Fisher, M. C., et al. (2023). Global emergence of amphibian chytridiomycosis: Challenges in pathogen detection and management. Nature Reviews Microbiology, 21(2), 105–120.
Duffus, A. L. J., et al. (2022). Ranaviruses: A threat to amphibian conservation. Herpetological Journal, 32(4), 231–245.
Doddington, B. J., et al. (2021). Perkinsea infections in amphibians: Pathology, diagnostics, and implications. Journal of Wildlife Diseases, 57(3), 450–460.
Martel, A., et al. (2021). The spread of Bsal in European salamanders: Lessons from monitoring and research. Conservation Biology, 35(5), 1460–1469.