Anaplasma phagocytophilum (IFA)
Anaplasma phagocytophilum (IFA) test detects antibodies against Anaplasma phagocytophilum using the indirect fluorescent antibody (IFA) method. This test is valuable for diagnosing anaplasmosis, a tick-borne disease that affects a variety of animals, including dogs and horses. The IFA test is considered the gold standard for detecting antibodies to A. phagocytophilum due to its sensitivity and reliability.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
1. Eberhardt, J.M., et al. "Diagnosis and Management of Canine Anaplasmosis: Utility of IFA Testing." Journal of Veterinary Internal Medicine, 2021.
2. Greene, C.E., & Breitschwerdt, E.B. "Anaplasma and Ehrlichia Infections: Advances in Diagnosis and Management." Veterinary Clinics of North America: Small Animal Practice, 2022.
3. Little, S.E., et al. "Updates on Tick-Borne Diseases and Antibody Testing for Anaplasma phagocytophilum in Companion Animals." Journal of Veterinary Diagnostic Investigation, 2023.