Anaplasma phagocytophilum (IFA) -Equine
The Anaplasma phagocytophilum IFA test is a diagnostic tool used to detect antibodies against Anaplasma phagocytophilum, the causative agent of equine granulocytic anaplasmosis (EGA). This test employs the indirect fluorescent antibody (IFA) method, recognized for its sensitivity in identifying exposure or active infection. EGA is a tick-borne disease transmitted primarily by Ixodes species, causing symptoms in horses such as fever, lethargy, limb edema, petechiae, ataxia, and in severe cases, neurological signs. Detection of antibodies to A. phagocytophilum via the IFA test supports diagnosis, particularly in endemic areas or in horses with clinical signs consistent with the disease. Early detection allows for timely treatment, improving recovery outcomes and reducing complications.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Pusterla, N., et al. "Clinical Features and Diagnostic Advances in Equine Granulocytic Anaplasmosis." Journal of Veterinary Internal Medicine, 2021.
Madigan, J.E., et al. "Tick-Borne Diseases in Horses: Diagnostic Tools and Treatment Approaches." Veterinary Clinics of North America: Equine Practice, 2022.
Gaffney, P.M., et al. "Evaluation of IFA for Detecting Anaplasma phagocytophilum in Equine Patients." Journal of Veterinary Diagnostic Investigation, 2023.