Anti-Müllerian Hormone (ELISA)
Anti-Mullerian Hormone (AMH) and inhibin testing are essential diagnostic tools for assessing reproductive health and functionality in horses. AMH is a glycoprotein hormone secreted by ovarian granulosa cells in mares and Sertoli cells in stallions, while inhibin is produced by granulosa cells and plays a role in regulating follicle-stimulating hormone (FSH) levels. Measuring these hormones provides valuable insights into ovarian and testicular function, particularly in diagnosing granulosa-theca cell tumors (GCTs) in mares. Elevated levels of AMH and inhibin are strongly indicative of GCTs, the most common ovarian tumors in mares. This combined testing approach enhances diagnostic accuracy, allowing for early detection and management. In stallions, AMH testing can differentiate between cryptorchid and castrated animals, while inhibin levels are less commonly used in male reproductive evaluations.mAMH and inhibin testing are highly specific, minimally invasive, and reliable, making them ideal for equine reproductive assessments. Early detection of abnormalities enables timely intervention, improving health outcomes and breeding efficiency.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Place, N.J., et al. "Use of Anti-Müllerian Hormone for Detection of Ovarian Remnant Syndrome and Spay Status in Dogs." Journal of Veterinary Diagnostic Investigation, 2022.
Romagnoli, S., & Concannon, P.W. "Reproductive Endocrinology and AMH Testing in Canine Practice." Theriogenology, 2023.
England, G.C.W., et al. "Clinical Applications of Anti-Müllerian Hormone in Veterinary Reproduction." Veterinary Reproductive Science, 2021.