Antinuclear Antibody (IFA)
The Antinuclear Antibody (ANA) test, conducted via Indirect Immunofluorescence Assay (IFA), is an essential diagnostic tool for identifying autoimmune diseases in dogs, particularly systemic lupus erythematosus (SLE) and other immune-mediated conditions. ANAs are autoantibodies that target components within the cell nucleus, and their presence often indicates an abnormal immune response. This test uses a fluorescent-tagged antibody to detect ANA in the blood, providing a sensitive method for confirming autoimmune activity. ANA testing is useful in cases where dogs present with symptoms suggestive of autoimmune disorders, including polyarthritis, skin lesions, and renal or blood abnormalities.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum.
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accept
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
1. Monroe, W.E., et al. "Diagnostic Approach to Autoimmune Diseases in Dogs: Utility of ANA Testing." Veterinary Clinics of North America: Small Animal Practice, 2022.
2.Day, M.J., et al. "Understanding Autoantibodies in Canine Systemic Autoimmune Disorders." Journal of Veterinary Internal Medicine, 2021.
3.Werner, M., et al. "The Role of Antinuclear Antibody Testing in Veterinary Medicine." Veterinary Immunology and Immunopathology, 2023.