Aspergillus spp. (AGID) - Equine
The Aspergillus spp. AGID test is a diagnostic tool used to detect antibodies against Aspergillus species, which are opportunistic fungal pathogens responsible for equine aspergillosis. This test uses the agar gel immunodiffusion (AGID) method to identify exposure or infection, providing a reliable assessment of fungal involvement in respiratory and systemic diseases. Aspergillus infections in horses commonly manifest as guttural pouch mycosis, which can lead to severe clinical signs such as nasal discharge, epistaxis (nosebleeds), dysphagia (difficulty swallowing), and, in some cases, neurological symptoms due to cranial nerve involvement. The AGID test is particularly valuable in diagnosing guttural pouch mycosis and systemic aspergillosis, especially in horses presenting with chronic respiratory symptoms or those residing in high-risk environments with exposure to moldy hay or damp conditions. Early detection through AGID testing facilitates timely intervention, reducing the risk of life-threatening complications.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Perkins, J.D., et al. "Equine Guttural Pouch Mycosis: Diagnostic Challenges and Advances." Journal of Equine Veterinary Science, 2021.
Sykes, J.E., et al. "Diagnostic Applications of Serological Testing for Aspergillosis in Equines." Veterinary Clinics of North America: Equine Practice, 2022.
Barratt, J.G., et al. "Evaluation of Agar Gel Immunodiffusion for Detecting Aspergillus spp. in Horses." Journal of Veterinary Diagnostic Investigation, 2023.