Dirofilaria immitis Antigen (ELISA)
The Dirofilaria immitis Antigen ELISA is a highly sensitive and specific immunoassay designed to detect circulating antigens of Dirofilaria immitis, the causative agent of heartworm disease. This test identifies adult female D. immitis worms by detecting the antigen released into the bloodstream, making it a critical tool for diagnosing heartworm infections.The ELISA (Enzyme-Linked Immunosorbent Assay) utilizes monoclonal antibodies to bind specifically to D. immitis antigens. This method allows for the detection of even low antigen concentrations, ensuring early and accurate diagnosis.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Vezzoni, A., et al. (2021). Advances in Heartworm Diagnostics and Management. Journal of Veterinary Parasitology, 287, 109-121.
Bowman, D. D., et al. (2020). Heartworm Biology, Treatment, and Control: An Update for Practitioners. Veterinary Clinics of North America: Small Animal Practice, 50(2), 271-287.
American Heartworm Society (2022). Current Guidelines for the Diagnosis, Prevention, and Management of Heartworm Disease in Dogs and Cats. Available at:www.heartwormsociety.org