Equine Infectious Anemia Virus (AGID)
The Equine Infectious Anemia Virus (EIA) Agar Gel Immunodiffusion (AGID) test, commonly referred to as the Coggins test, is a gold-standard serological assay used to detect antibodies against Equine Infectious Anemia Virus (EIAV) in equine blood. EIAV is a retrovirus that causes equine infectious anemia (EIA), a potentially fatal disease characterized by recurring episodes of fever, anemia, weight loss, and edema. Infected horses often become lifelong carriers, posing a risk of transmission to others through blood-feeding insects like horseflies and deerflies. The AGID test is highly reliable and widely accepted for regulatory purposes, including interstate and international transport, sale, and shows. It identifies antibodies indicative of chronic or past infections, even in asymptomatic carriers. Early detection of EIAV-infected animals through AGID testing helps veterinarians and regulatory authorities implement quarantine, culling, or management measures to control the spread of the disease and safeguard equine populations.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Issel, C.J., et al. "Advances in Equine Infectious Anemia Virus Diagnostics and Management." Journal of Equine Veterinary Science, 2021.
Cook, R.F., et al. "The Role of AGID Testing in Controlling Equine Infectious Anemia: An Overview." Veterinary Clinics of North America: Equine Practice, 2022.
Leroux, C., et al. "Serological Testing for EIAV: Implications for Equine Health and Biosecurity." Journal of Veterinary Diagnostic Investigation, 2023.