Equine Infectious Disease Serology Panel
The Equine Infectious Disease Serology Panel is a comprehensive diagnostic tool designed to detect antibodies against a range of pathogens responsible for significant equine diseases. This panel utilizes advanced immunological techniques, including Indirect Fluorescent Antibody (IFA) and Enzyme-Linked Immunosorbent Assays (ELISA), to provide accurate and reliable serological results from equine serum samples. This panel evaluates exposure or immune response to the following pathogens Neorickettsia risticii (Potomac Horse Fever) detected using IFA, this pathogen causes Potomac Horse Fever, characterized by fever, diarrhea, and laminitis. Theileria equi detected using ELISA, it is a protozoan parasite causing equine piroplasmosis, leading to fever, anemia, and jaundice. Babesia caballi also detected using ELISA, another cause of equine piroplasmosis, producing similar clinical signs. Sarcocystis neurona detects antibodies using ELISA to this protozoan, the causative agent of Equine Protozoal Myeloencephalitis (EPM), a neurologic disease with symptoms like ataxia and muscle wasting. Streptococcus equi ELISA identifies exposure to this bacterium, which causes strangles, a highly contagious respiratory disease. West Nile Virus (WNV) IgM ELISA detects antibodies associated with recent exposure to WNV, a mosquito-borne virus causing encephalitis and neurologic signs.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Knowles, D. P., & Kappmeyer, L. S. (2018). Advances in Diagnostic Testing for Equine Piroplasmosis. Journal of Veterinary Diagnostic Investigation, 30(2), 165–176.
Dubey, J. P., & Howe, D. K. (2020). Sarcocystis neurona and Equine Protozoal Myeloencephalitis: An Overview. Veterinary Parasitology, 277, 109013.
Timoney, J. F. (2016). The Biology of Streptococcus equi: An Update. Equine Veterinary Journal, 48(4), 395–400.
Long, M. T., & Ostlund, E. N. (2019). Diagnostic Advances in West Nile Virus and Neurologic Diseases in Horses. Journal of Veterinary Internal Medicine, 33(2), 695–703.