Protein Electrophoresis - Equine
Protein electrophoresis is a diagnostic laboratory technique used to separate and quantify the different protein fractions in equine serum samples. This method provides valuable insights into the composition and balance of proteins, helping to diagnose and monitor various diseases in horses. The primary protein fractions include albumin and globulins, with the latter further divided into alpha, beta, and gamma globulins. In equines, protein electrophoresis is particularly useful for diagnosing inflammatory, infectious, and neoplastic conditions, as well as assessing overall health. Elevated gamma globulin levels may indicate chronic inflammation or immune stimulation, while low albumin levels can suggest liver dysfunction, protein-losing nephropathy, or gastrointestinal diseases.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label.
Ensure the submittal form is completed with the animal’s information and relevant medical history.
McKenzie, E.C., et al. "Clinical Applications of Protein Electrophoresis in Equine Medicine." Journal of Equine Veterinary Science, 2021.
Toribio, R.E., et al. "Serum Protein Analysis and Its Role in Diagnosing Equine Diseases." Veterinary Clinics of North America: Equine Practice, 2022.
Hinchcliff, K.W., et al. "Advances in Equine Laboratory Diagnostics: Protein Electrophoresis Applications." Journal of Veterinary Diagnostic Investigation, 2023.