Streptococcus equi (ELISA) - Equine
The Streptococcus equi ELISA (Enzyme-Linked Immunosorbent Assay) test is a sensitive diagnostic tool designed to detect antibodies against Streptococcus equi subspecies equi, the causative agent of strangles in horses. Strangles is a highly contagious bacterial infection characterized by fever, nasal discharge, lymph node abscessation, and respiratory distress. The ELISA test is particularly valuable for identifying horses that have been exposed to the pathogen, including asymptomatic carriers, which are critical in the spread of the disease.This test measures the horse’s immune response to specific S. equi proteins, providing veterinarians with insight into recent or past exposure to the bacterium. It is especially useful for screening horses in outbreak situations, pre-purchase examinations, or for monitoring horses in high-risk environments. Additionally, the Streptococcus equi ELISA test supports post-treatment surveillance to ensure resolution of the infection. When paired with other diagnostic methods, such as PCR or bacterial culture, it enhances the accuracy of diagnosis and helps guide control measures to prevent further transmission.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Waller, A.S., et al. "Advances in Serological Testing for Streptococcus equi: The Role of ELISA." Journal of Equine Veterinary Science, 2021.
Pusterla, N., et al. "Clinical Applications of ELISA in Diagnosing and Managing Strangles." Veterinary Clinics of North America: Equine Practice, 2022.
Timoney, J.F., et al. "Serological and Molecular Diagnostics for Strangles in Equine Populations." Journal of Veterinary Diagnostic Investigation, 2023.