Toxoplasma gondii IgG & IgM Antibody Panel (IFA) - Equine
The Toxoplasma gondii IgG and IgM Panel, performed using indirect fluorescent antibody (IFA) testing, is a serological diagnostic tool used to detect antibodies against Toxoplasma gondii in equines. This test differentiates between acute and past exposure by measuring both IgG and IgM antibody levels. IgM indicates recent or active infection, while IgG suggests prior exposure or chronic infection. Although horses are considered relatively resistant to clinical toxoplasmosis, they can act as intermediate hosts and may occasionally exhibit systemic or neurological signs under certain conditions, such as immunosuppression.The IgG and IgM panel is particularly valuable for diagnosing rare cases of T. gondii infection in equines or for screening populations in areas where the parasite is endemic. The test is performed on serum and aids in distinguishing T. gondii infections from other conditions with similar clinical signs.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Dubey, J.P., et al. "Serological Diagnosis of Toxoplasma gondii Infections in Equines: Applications of IgG and IgM Antibody Testing." Journal of Equine Veterinary Science, 2021.
Hinchcliff, K.W., et al. "Advances in Diagnostic Methods for Equine Protozoal Infections: The Role of IgG and IgM Panels for T. gondii." Veterinary Clinics of North America: Equine Practice, 2022.
Pusterla, N., et al. "Clinical Utility of IgG and IgM Antibody Testing for Diagnosing Toxoplasma gondii in Horses." Journal of Veterinary Diagnostic Investigation, 2023.