Toxoplasma gondii (rtPCR)
The Toxoplasma gondii (rtPCR) test uses real-time polymerase chain reaction (rtPCR) to detect Toxoplasma gondii DNA, a protozoan parasite that can infect both animals and humans, posing significant zoonotic risks. T. gondii infections in animals, particularly in cats (the primary host), can lead to various clinical symptoms, including lethargy, fever, respiratory distress, and neurological signs, although many animals remain asymptomatic. This test provides highly sensitive and specific detection, even at low levels, enabling early and accurate diagnosis. The rtPCR method is particularly useful for diagnosing active infections in symptomatic animals or monitoring infection status in multi-animal environments,
5g fecal sample
Ideally, fecal samples should be taken directly from the rectum. If samples are collected from the ground, they should be taken from the surface of a freshly deposited stool.
An appropriate sample size is 5g in a leak proof sterile container.
Preferably submit the sterile container in a zip-lock type plastic bag
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Dubey, J.P., et al. (2022). "Advances in Toxoplasma gondii Detection and Management in Veterinary Medicine." Veterinary Clinics of North America: Small Animal Practice.
Lappin, M.R., et al. (2023). "Real-Time PCR for Toxoplasma gondii: Applications and Clinical Relevance." Journal of Veterinary Diagnostic Investigation.
Hill, D., et al. (2021). "Diagnosis and Zoonotic Potential of Toxoplasmosis in Companion Animals." Journal of Feline Medicine and Surgery.