Trypsin-Like Immunoreactivity (TLI) - Feline
The Trypsin-Like Immunoreactivity (TLI) test is a specialized diagnostic tool used to assess pancreatic function in dogs, particularly to diagnose exocrine pancreatic insufficiency (EPI). TLI measures serum concentrations of trypsinogen and trypsin, enzymes produced by the pancreas, which are typically present at low levels in healthy dogs. Elevated TLI levels may indicate pancreatitis, while significantly reduced levels are indicative of EPI, a condition where the pancreas fails to produce sufficient digestive enzymes.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum.
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accept
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Steiner, J.M., et al. "Evaluation of Serum Trypsin-Like Immunoreactivity in Feline Exocrine Pancreatic Insufficiency." Journal of Veterinary Internal Medicine, 2022.
Simpson, K.W., & Steiner, J.M. "Diagnostic Techniques in Feline Pancreatic Disorders: The Role of TLI Testing." Veterinary Clinics of North America: Small Animal Practice, 2021.
Forman, M.A., et al. "Trypsin-Like Immunoreactivity in Cats: Diagnostic Utility and Clinical Applications." Journal of Feline Medicine and Surgery, 2023.