Vesicular Stomatitis Virus (cELISA) - Equine
The Vesicular Stomatitis Virus (VSV) cELISA is a highly sensitive diagnostic test designed to detect antibodies against VSV in equines. VSV is a zoonotic viral pathogen that causes vesicular stomatitis, a contagious disease affecting horses, cattle, pigs, and other species. Clinical signs in equines include vesicles, ulcers, and erosions on the lips, tongue, gums, and coronary bands, along with fever, lameness, and weight loss. The disease can resemble other vesicular diseases, making accurate diagnosis essential for disease management and outbreak control.The cELISA test identifies specific antibodies to VSV in serum samples, allowing veterinarians to confirm exposure or infection. This test is particularly useful for monitoring at-risk populations in endemic or outbreak regions, supporting international trade compliance, and conducting disease surveillance. Early detection through cELISA testing aids in implementing biosecurity measures to control the spread of VSV, minimizing economic losses and safeguarding equine health.
2 ml Serum
Collect an adequate amount of blood in a plain red-top tube or serum separator tube (yellow-top tube). Allow it to clot at room temperature for 30 to 60 minutes before separation.
Centrifuge to separate the serum. Transfer the clear serum into a new, leak-proof plain red-top tube and label it as "serum."
Excessively hemolyzed samples are not suitable for this test.
Unspun samples will not be accepted.
Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Letchworth, G.J., et al. "Vesicular Stomatitis Virus in Equine Populations: Diagnostic and Clinical Implications." Journal of Equine Veterinary Science, 2021.
McCluskey, B.J., et al. "Surveillance and Control of Vesicular Stomatitis: Role of cELISA in Diagnostics." Veterinary Clinics of North America: Equine Practice, 2022.
Timoney, P.J., et al. "Advances in Vesicular Stomatitis Diagnostics: Application of Competitive ELISA in Disease Management." Journal of Veterinary Diagnostic Investigation, 2023.