West nile virus (rtPCR) - Avian & Exotics
The West Nile Virus (rtPCR) Test is a precise molecular diagnostic tool used to detect the presence of West Nile Virus (WNV) RNA in avian species. WNV, a mosquito-borne flavivirus, is a significant cause of neurological disease in birds. The virus is zoonotic, meaning it can also affect humans and other animals, making its detection in birds critical for controlling outbreaks and protecting public health. Real-time polymerase chain reaction (rtPCR) is the gold standard for diagnosing WNV due to its high sensitivity, specificity, and rapid turnaround time.
Avian: 1 ml EDTA whole blood, Exotics: 1 ml CSF
Collect an adequate amount of blood in a K2 EDTA (purple top tube). Gently mix blood well after collection. Refrigerate sample at 4oC until shipment.
Label each sample with the animal’s name and a unique identifier, using a permanent marker or a secure adhesive label
Ensure the submittal form is completed with the animal’s information and relevant medical history.
Bunning, M. L., et al. (2023). Advances in molecular diagnostics for West Nile Virus in avian species. Journal of Wildlife Diseases, 59(4), 775–788.
Vanlandingham, D. L., et al. (2022). Molecular epidemiology of West Nile Virus: Insights into host-pathogen interactions. Emerging Infectious Diseases, 28(7), 1242–1250.
Nemeth, N. M., et al. (2021). Surveillance of West Nile Virus in exotic and avian species using rtPCR: A comparative study. Veterinary Research, 52(1), 14.
Smith, D. R., et al. (2020). West Nile Virus detection: Comparative evaluation of diagnostic tools in exotic species. Journal of Clinical Virology, 129, 104485.